Composite

Part:BBa_K3016210:Design

Designed by: Antti Pennanen, Antti Toivanen, Amanda Raitosalo   Group: iGEM19_Aalto-Helsinki   (2019-10-16)


Vibrio natriegens' TorA signal peptide containing human growth hormone with 6xHis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 515
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

hGH has one CTG codon changed to CTA in order to avoid a illegal PstI site. For more information see part BBa_K3016500.


Source

TorA signal sequence from Vibrio natriegens ATCC 14048 / DSM 759 genome, hGH gene with His tag from Browning et al., 2017 (see references).

References

Browning, D. F., Richards, K. L., Peswani, A. R., Roobol, J., Busby, S. J., & Robinson, C. (2017). Escherichia coli “TatExpress” strains super‐secrete human growth hormone into the bacterial periplasm by the Tat pathway. Biotechnology and bioengineering, 114(12), 2828-2836.

Gunawardane, K., Hansen, T. K., Christiansen, J. S., & Jorgensen, J. O. L. (2015). Normal physiology of growth hormone in adults. In Endotext [Internet]. MDText. com, Inc..